A TUNEL assay (KeyGen Biotech, China, Nanjing) was performed according to a modification of a published method 24. Sections stained without biotinylated dUTP were used as negative controls.
For cell cycle analysis, the cells were fixed in 70% ice-cold ethanol for 2 h and stained with propidium iodide (PI, Keygen, Jiangsu, China) in the presence of RNase A (Qiagen, Germany). Fluorescence.
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People go from the enormous high of flow (considered the most addictive feeling on earth) to a crashing low that follows. This low results from the fact that the brain has temporarily used up its.
Dihydroethidium (DHE) was obtained from Keygen Biotech, and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), malondialdehyde (MDA) assay kit, glutathione (GSH) assay kit, total SOD assay kit and.
ROS-mediated inactivation of the PI3K/AKT pathway is involved in the antigastric cancer effects of thioredoxin reductase-1 inhibitor chaetocin – The results were analyzed using the real-time cell analysis software supplied by the company. A cell counting kit-8 (CCK-8) assay (Nanjing KeyGen Biotech Co., Ltd.) was used to analyze the effect of.
After different treatments and incubation for 14 h, the cells were collected with a concentration of 1 × 10 6 cells/mL and the suspension was detected with a flow cytometer, according to the.
Cells were harvested, fixed with 70% ethanol overnight at 4 °C, and stained with propidium iodide (KeyGEN, Jiangsu, China) in the dark for 1 h. Cell suspensions were subjected to flow cytometry with.
DNase-1 Treatment Exerts Protective Effects in a Rat Model of Intestinal Ischemia-Reperfusion Injury – Detection of IL-6, IL-10 and tumor necrosis factor-alpha by ELISA The IL-6, IL-10 and tumor necrosis factor-alpha (TNF-α) levels in the serum, which reflect the severity of inflammation, were measured.